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The species of yeast used to make pale lager, Saccharomyces carlsbergensis, was isolated by Emil Christian Hansen at the laboratory in 1883 and bears its name; this was shared freely by Carlsberg. Because of a conflict with his son Carl, Jacobsen's brewery was left to the Foundation upon his death in 1887.

The first overseas license for brewing was given to the Photos Photiades Breweries, and in 1966 Carlsberg glass and beer was brewed for the first time outside Denmark at the Photiades breweries in Cyprus.

Jacobsen set up the Carlsberg Laboratory in 1875, which worked on scientific problems related to brewing.

It featured a Department of Chemistry and a Department of Physiology. Jacobsen established the Carlsberg Foundation, run by trustees from the Royal Danish Academy of Sciences and Letters, which managed the Carlsberg Laboratory as well as supporting scientific research within the fields of natural sciences, mathematics, philosophy, the humanities and social sciences in Denmark.

Jacobsen, the company's headquarters is located in Copenhagen, Denmark.

Some of the company's original logos include an elephant, after which some of its lagers are named, and the swastika whose use was discontinued in the 1930s because of its association with political parties in neighboring Germany.

Jacobsen's son Carl opened a brewery in 1882 named Ny (New) Carlsberg forcing him to rename his brewery Gamle (Old) Carlsberg.

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It has a majority interest in OAO Baltika Breweries, the largest brewery in the Russian Federation and Eastern Europe.In August 2011, Carlsberg announced a new joint venture with Chongqing Brewery and Chongqing Light Textile Holding.